NPM1 Antibody (NPM1/3285) [FITC]

• Reactivity: Hu
• Applications: ELISA, Endotoxin, ICC/IF, IHC
• Clone: NPM1/3285
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

NPM1 Antibody (NPM1/3285) [FITC] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary) (Uniprot: P06748)
• Localization: Nuclear and Cytoplasmic
• Marker: Acute Myeloid Leukemia Marker
• Specificity: Recognizes a 33kDa glycoprotein, identified as NPM1 (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-08745. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for NPM1 Antibody (NPM1/3285) [FITC]

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPM1