NOTUM Antibody - BSA Free

Western Blot: NOTUM Antibody [NBP2-66631]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NOTUM Antibody - BSA Free Summary

• Immunogen: Rabbits were immunized with recombinant full-length notum protein from mouse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOTUM
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 5 - 10 ug/mL
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for NOTUM Antibody - BSA Free

• EC 3.-
• notum pectinacetylesterase homolog (Drosophila)
• Notum
• protein notum homolog

Background

Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the / -hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NOTUM Antibody (NBP2-66631). (Showing 1 - 1 of 1 FAQ).

1.  is there information available to know if Novus item NBP2-66631 binds to R&D item 9150-NO-050? I am looking to build a sandwich ELISA and am on the hunt for potential antibody pairs and a standard material
   • So while we have not used NBP2-66631 explicitly with 9150-NO-050, it should work to detect this product as the AB was raised against recombinante full length Mouse Notum protein, and R&D's protein is Ser88-Thr458 (Cys337Ser) with a C-terminal 6-His tag. As a polyclonal AB and mouse reactive, this should be a good product to consider. However, we have not validated this AB for use in a sandwich ELISA, so it is not covered in our guarantee.

Bioinformatics

• Gene Symbol: NOTUM