NOTUM Antibody - BSA Free

Images

 
Western Blot: NOTUM Antibody [NBP1-77060] - Human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: NOTUM Antibody [NBP1-77060] - Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: NOTUM Antibody [NBP1-77060] - Human brain tissue with NOTUM antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

NOTUM Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM. Amino Acid Squence: CNDGSPAGYYLKESRGSRR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOTUM
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1ug/ml
Control Peptide
NOTUM Peptide (NBP1-77060PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for NOTUM Antibody - BSA Free

  • EC 3.-
  • notum pectinacetylesterase homolog (Drosophila)
  • Notum
  • protein notum homolog

Background

In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NOTUM
Uniprot