NOP58 Antibody (34B12)

Western Blot: NOP58 Antibody [NBP1-05225] - Western Blot analysis of whole yeast protein extracts detect NOP58. Molecular weight detected is 57 kDa.

Product Discontinued

NOP58 Antibody (34B12) Summary

• Immunogen: A yeast nucleolar preparation
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOP58
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:10000
  • Immunoprecipitation 2-4 ul
  • Western Blot 1:10000
• Application Notes: This NOP58 (34B12) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at 57 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated hybridoma cell culture media.
• Preservative: 0.035% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for NOP58 Antibody (34B12)

• HSPC120
• NOL5
• NOP5/NOP58
• NOP58 ribonucleoprotein homolog (yeast)
• NOP5nucleolar protein 58
• Nucleolar protein 5
• nucleolar protein NOP5/NOP58

Background

NOP58 (nucleolar protein 58) is a member of the conserved nop5 (Drosophila)/sik1/Nop56p (yeast) gene family that encodes components of snoRNP/small nucleolar ribonucleoproteins, which contains a snoRNA and a complex of proteins. NOP58 belongs to box C/D snoRNP, which is named for short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. snoRNP primarily catalyzes the cleavage as well as modification of rRNA during the biogenesis of ribosomes, nucleolar sizing , and snoRNA trafficking . In yeast, at least two members, Nop56 and Nop58, are present, and yeast cells deficient in either of the two members undergoes growth arrest because of defective protein translation. Furthermore, functional mutations of Nop56p/sik1 have been shown to cause reduced cell growth and proliferation. In mammalian cells, NOP58 has been found to increase rRNA synthesis and translational efficiency of the exogenous luciferase (Luc) gene, and its expression correlate with metastatic phenotypes of clinical melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOP58