NONO Antibody

Images

 
Western Blot: NONO Antibody [NB100-40789] - Analysis of NONO using this antibody at 0.1 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NONO Antibody [NB100-40789] - HEK293 overexpressing Human NONO and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NONO Antibody Summary

Immunogen
Peptide with sequence C-NRAAPGAEFAPNK corresponding to C-Terminus according to NP_031389.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NONO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 1:200
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 60 kDa band observed in mouse brain lysates (calculated MW of 54.2 kDa band according to human NP_031389.3 and 54.5 kDa band according to mouse NP_075633.2). In transfected HEK293 transiently expressing human NONO a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 50 kDa band protein is observed in both. IHC-P: Human spleen shows nuclear staining in select splenocytes.
Positive Control
NONO Lysate (NBL1-13717)
Publications
Read Publication using NB100-40789.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NONO Antibody

  • NMT5552 kDa subunit
  • non-POU domain containing, octamer-binding
  • NRB54non-POU-domain-containing, octamer-binding
  • p54(nrb)

Background

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NONO Antibody (NB100-40789)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

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Bioinformatics

Gene Symbol NONO
Entrez
Uniprot