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Nogo-B Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
AF6034A
Biotin
AF6034B
CoraFluor 1
AF6034CL1
DyLight 350
AF6034D
DyLight 488
AF6034K
DyLight 550
AF6034L
DyLight 594
AF6034M
DyLight 650
AF6034W
DyLight 680
AF6034Y
DyLight 755
AF6034Z
FITC
AF6034F
HRP
AF6034H
Janelia Fluor 525
AF6034JF525
Janelia Fluor 549
AF6034I
Janelia Fluor 646
AF6034J
Janelia Fluor 669
AF6034JF669
PE
AF6034P
PerCP
AF6034C
Unconjugated
AF6034
mFluor Violet 450 SE
AF6034MFV450
mFluor Violet 500 SE
AF6034MFV500
mFluor Violet 610 SE
AF6034MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF6034
Carrier Free
AF6034
Carrier Free
AF6034-SP
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Nogo-B Antibody [DyLight 405] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Nogo-B
Met1-Val200
Accession # NP_722550
Specificity
Detects human and mouse Nogo-B in direct ELISAs and Western blots.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
RTN4
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Nogo-B Antibody [DyLight 405]

  • ASY;Nbla00271;Nbla10545;NI220/250;NOGO;NSP;NSP-CL;Reticulon-4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X
  • NogoB
  • Nogo-B
  • RTN4B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Nogo-B Antibody
[DyLight 405]
AF6034E
Nogo-B Antibody [DyLight 405]
Species: Hu, Mu
Applications: WB, IHC

Publications for Nogo-B Antibody (AF6034E) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Isotype Controls

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NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Nogo Receptor/NgR Antibody
AF1440

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Bioinformatics

Gene Symbol RTN4