Nodal Antibody (784410) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clone: 784410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Nodal Antibody (784410) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Nodal
  His238-Leu347
  Accession # Q96S42
• Specificity: Detects human Nodal in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Nodal is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nodal Antibody (784410) [Alexa Fluor® 700]

• BMP-16
• MGC138230
• nodal homolog (mouse)
• nodal homolog
• Nodal
• nodal, mouse, homolog

Background

Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34-39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the type I receptor ALK7. Nodal interacts extracellularly with members of other protein families including Cerberus, Lefty, and EGF-CFC ligands such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.