nNOS Antibody (85327) [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 85327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

nNOS Antibody (85327) [Alexa Fluor® 532] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
  Ser218-Ser1434
  Accession # P29475
• Specificity: Detects human nNos in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for nNOS Antibody (85327) [Alexa Fluor® 532]

• bNOS
• brain
• Constitutive NOS
• EC 1.14.13.39
• IHPS1
• Neuronal NOS
• nitric oxide synthase 1 (neuronal)
• nNOS
• N-NOS
• nNOSNOS type I
• NOS
• NOS1
• Peptidyl-cysteine S-nitrosylase NOS1

Background

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. nNOS exists as homodimers containing a cytochrome P450‑like prosthetic heme group in the N-terminal half. It also has a tightly bound FAD and FMN group in the C-terminal half. At least 4 isoforms of human nNOS are known. Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. It also shares 96% sequence identity with mouse or rat nNOS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.