NMDAR2A Antibody (JA31-20)

Western Blot: NMDAR2A Antibody (JA31-20) [NBP2-66984] - Western blot analysis of NMDAR2A on mouse hippocampus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: NMDAR2A Antibody (JA31-20) [NBP2-66984] - Analysis of paraffin-embedded rat skin tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.

Western Blot: NMDAR2A Antibody (JA31-20) [NBP2-66984] - Analysis of NMDAR2A on rat brain tissue lysate using anti-NMDAR2A antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: NMDAR2A Antibody (JA31-20) [NBP2-66984] - Analysis of paraffin-embedded human brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NMDAR2A Antibody (JA31-20) [NBP2-66984] - Analysis of paraffin-embedded mouse small intestine tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NMDAR2A Antibody (JA31-20) [NBP2-66984] - Analysis of paraffin-embedded rat brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.

Product Discontinued

NMDAR2A Antibody (JA31-20) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human NMDAR2A aa 1356-1405 / 1464. (SwissProt: Q12879 Human; SwissProt: P35436 Mouse; SwissProt: Q00959 Rat)
• Localization: Cell membrane, postsynaptic cell membrane, dendritic spine, synapse, Cytoplasmic vesicle membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GRIN2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NMDAR2A Antibody (JA31-20)

• GluN2A
• glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• hNR2A
• NMDA receptor subtype 2A
• N-methyl D-aspartate receptor subtype 2A
• N-methyl-D-aspartate receptor subunit 2A
• subunit epsilon-1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN2A