NISCH Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

NISCH Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human NISCH.
  
  

  Source: E. coli

  Amino Acid Sequence: AHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEV

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: NISCH
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-81630.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for NISCH Recombinant Protein Antigen

• hIRAS
• I-1 receptor candidate protein
• I-1
• I-1FLJ14425
• I1R candidate protein
• I1R
• Imidazoline receptor 1
• imidazoline receptor antisera selected
• Imidazoline receptor antisera-selected protein
• imidazoline receptor candidate
• Imidazoline-1 receptor candidate protein
• Imidazoline-1 receptor
• IR1
• IRAS
• IRASFLJ40413
• KIAA0975
• KIAA0975FLJ90519
• NISCH
• nischarin

Background

NISCH (Nischarin) is involved in several biological processes including cell communication, actin cytoskeleton organization, regulation of synaptic transmission and negative regulation of cell migration. NISCH is known to have interactions with AR, IRS2, ITGA5, IRS1 and IRS4. Disease and disorder research has been conducted in relation to NISCH and high blood pressure, blindness, Alzheimer's disease, brain disease, breast cancer, rosacea, hypertension and pancreatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NISCH