NIPP1 Antibody

Western Blot: NIPP1 Antibody [NB100-1286] - (1ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286] - 3.8ug/ml staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NIPP1 Antibody Summary

• Description: Novus Biologicals Goat NIPP1 Antibody (NB100-1286) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-NIPP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EAWPGKKPTPSLLI corresponding to C-Terminus according to NP_002704.1, NP_054829.2, NP_612568.1.
• Specificity: This antibody is expected to recognise all three human isoforms of this protein.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP1R8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 2 ug/ml
• Application Notes: Western blot: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.
  IHC: In paraffin embedded Human Thymus shows speckled staining of nuclei.
• Control:

  NIPP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NIPP1 Antibody

• activator of RNA decay
• ARD1
• ard-1
• ARD1nuclear inhibitor of protein phosphatase 1
• NIPP1
• NIPP1nuclear inhibitor of protein phosphatase-1
• NIPP-1nuclear subunit of PP-1
• PPP1R8
• PRO2047
• Protein phosphatase 1 regulatory inhibitor subunit 8
• protein phosphatase 1 regulatory subunit 8
• protein phosphatase 1, regulatory (inhibitor) subunit 8
• RNase E

Background

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIPP1 Antibody (NB100-1286)(2)

Publications using NB100-1286

• Nuytten M, Beke L, Van Eynde A, Ceulemans H, Beullens M, Van Hummelen P, Fuks F, Bollen M. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene;27(10):1449-60. 2008-02-28 [PMID: 17724462]
• Beullens M, Bollen M. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem 2002-05-31 [PMID: 11909864]

Bioinformatics

• Gene Symbol: PPP1R8
• Entrez:
  • 5511(Human)
  • 100336(Mouse)
• Uniprot:
  • NP_002704.1(Human)