NIPP1 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

NIPP1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human NIPP1
  Ala2-Ile351
  Accession # Q12972
• Specificity: Detects human, mouse, and rat NIPP1 in Western blots and detects recombinant human NIPP1 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NIPP1 Antibody [Alexa Fluor® 488]

• activator of RNA decay
• ARD1
• ard-1
• ARD1nuclear inhibitor of protein phosphatase 1
• NIPP1
• NIPP1nuclear inhibitor of protein phosphatase-1
• NIPP-1nuclear subunit of PP-1
• PPP1R8
• PRO2047
• Protein phosphatase 1 regulatory inhibitor subunit 8
• protein phosphatase 1 regulatory subunit 8
• protein phosphatase 1, regulatory (inhibitor) subunit 8
• RNase E

Background

NIPP1 (Nuclear Inhibitory polypeptide of Protein Phosphatase 1; also ARD-1 and PPP1R8) is a 41 kDa intracellular protein that belongs to no known molecular family. Although it is ubiquitously expressed, it does undergo alternative splicing, and distinct isoforms are associated with distinct tissues. In the nucleus, NIPP1 complexes with PP1c, targeting it to either RNA or chromatin. This is accompanied by the additional recruitment of other molecules such as Sap155 and PRC2. When nonphosphorylated, NIPP1 blocks PP1c activity; when phosphorylated, NIPP1 allows for PP1c activity, and this impacts the processes of both pre-mRNA splicing and gene silencing. Human NIPP1 is 351 amino acids (aa) in length. It contains an N-terminal FHA domain (aa 49-101), an NLS (aa 185-209), a PP1 binding site (aa 191-210), another NLS (aa 210-240) and an RNA binding site (aa 330-351). There are two potential splice variants. Both possess alternative start sites, one at Met143 (called NIPP1 beta ), and another at Met225 (called ARD-1 or NIPP1 gamma ). Full-length human NIPP1 shares 98% aa sequence identity with mouse NIPP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.