NIPP1 Antibody (4B5) - Azide and BSA Free

Western Blot: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 expression in transfected 293T cell line by PPP1R8 monoclonal antibody (M05), clone 4B5. Lane 1: PPP1R8 transfected lysate (Predicted MW: 38.5 KDa). Lane 2: Non-transfected lysate.

ELISA: NIPP1 Antibody (4B5) [H00005511-M05] - Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody.

Immunoprecipitation: NIPP1 Antibody (4B5) [H00005511-M05] - Analysis of PPP1R8 transfected lysate using anti-PPP1R8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPP1R8 monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 4B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NIPP1 Antibody (4B5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPP1R8 (NP_002704.1, 28 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
• Specificity: PPP1R8 - protein phosphatase 1, regulatory (inhibitor) subunit 8 (4B5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1R8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NIPP1 Antibody (4B5) - Azide and BSA Free

• activator of RNA decay
• ARD1
• ard-1
• ARD1nuclear inhibitor of protein phosphatase 1
• NIPP1
• NIPP1nuclear inhibitor of protein phosphatase-1
• NIPP-1nuclear subunit of PP-1
• PPP1R8
• PRO2047
• Protein phosphatase 1 regulatory inhibitor subunit 8
• protein phosphatase 1 regulatory subunit 8
• protein phosphatase 1, regulatory (inhibitor) subunit 8
• RNase E

Background

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R8
• Entrez:
  • 5511(Human)
• Uniprot:
  • NP_002704(Human)