NIPP1 Antibody (1G11) - Azide and BSA Free

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of PPP1R8 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of monoclonal antibody to PPP1R8 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of PPP1R8 expression in PC-12 (Cat # L012V1).

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of PPP1R8 expression in Hela S3 NE (Cat # L013V3).

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of PPP1R8 expression in Raw 264.7 (Cat # L024V1).

Sandwich ELISA: NIPP1 Antibody (1G11) [H00005511-M21] - Detection limit for recombinant GST tagged PPP1R8 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 1G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NIPP1 Antibody (1G11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPP1R8 (AAH13360, 1 a.a. ~ 209 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRMRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
• Specificity: Reacts with protein phosphatase 1, regulatory (inhibitor) subunit 8.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1R8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NIPP1 Antibody (1G11) - Azide and BSA Free

• activator of RNA decay
• ARD1
• ard-1
• ARD1nuclear inhibitor of protein phosphatase 1
• NIPP1
• NIPP1nuclear inhibitor of protein phosphatase-1
• NIPP-1nuclear subunit of PP-1
• PPP1R8
• PRO2047
• Protein phosphatase 1 regulatory inhibitor subunit 8
• protein phosphatase 1 regulatory subunit 8
• protein phosphatase 1, regulatory (inhibitor) subunit 8
• RNase E

Background

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R8
• Entrez:
  • 5511(Human)
• Uniprot:
  • AAH13360(Human)