Nidogen-2 Antibody (4G8)

Images

 
Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01] - Analysis of NID2 expression in transfected 293T cell line by NID2 monoclonal antibody (M01), clone 4G8.Lane 1: NID2 transfected lysate (Predicted MW: 107.1 ...read more
Sandwich ELISA: Nidogen-2 Antibody (4G8) [H00022795-M01] - Detection limit for recombinant GST tagged NID2 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Nidogen-2 Antibody (4G8) Summary

Immunogen
NID2 (NP_031387, 1276 a.a. ~ 1376 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.PNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK
Specificity
Reacts with nidogen 2 (osteonidogen).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NID2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Nidogen-2 Antibody (4G8)

  • NID2
  • NID-2
  • nidogen 2 (osteonidogen)
  • Nidogen2
  • Nidogen-2
  • Osteonidogen

Background

Basement membranes, which are composed of type IV collagens (see MIM 120130), laminins (see LAMC1; MIM 150290), perlecan (HSPG2; MIM 142461), and nidogen (see NID1; MIM 131390), are thin pericellular protein matrices that control a large number of cellular activities, including adhesion, migration, differentiation, gene expression, and apoptosis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NID2
Entrez
Uniprot