| Reactivity | MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-Fr |
| Clone | ELM1 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | DyLight 405 |
| Immunogen | Clone ELM1 is a rat monoclonal IgG2a antibody derived by fusion of X63 Ag 8.653 mouse myeloma cells with spleen cells from a Fisher rat immunized with a partially purified preparation of laminin from the EHS mouse tumor. |
| Specificity | ELM1 reacts with entactin. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | NID1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Nidogen-1/Entactin Antibody (ELM1) [DyLight 405]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Nidogen-1/Entactin Antibody (ELM1) [DyLight 405]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP1-97701AF350 | |
| Alexa Fluor 405 | NBP1-97701AF405 | |
| Alexa Fluor 488 | NBP1-97701AF488 | |
| Alexa Fluor 532 | NBP1-97701AF532 | |
| Alexa Fluor 594 | NBP1-97701AF594 | |
| Alexa Fluor 647 | NBP1-97701AF647 | |
| Alexa Fluor 700 | NBP1-97701AF700 | |
| Alexa Fluor 750 | NBP1-97701AF750 | |
| Biotin | NBP1-97701B | |
| DyLight 350 | NBP1-97701UV | |
| DyLight 405 | NBP1-97701V | |
| DyLight 488 | NBP1-97701G | |
| DyLight 550 | NBP1-97701R | |
| DyLight 594 | NBP1-97701DL594 | |
| DyLight 650 | NBP1-97701C | |
| DyLight 680 | NBP1-97701FR | |
| DyLight 755 | NBP1-97701IR | |
| FITC | NBP1-97701F | |
| HRP | NBP1-97701H | |
| Janelia Fluor 549 | NBP1-97701JF549 | |
| Janelia Fluor 646 | NBP1-97701JF646 |
Diseases for Nidogen-1/Entactin Antibody (NBP1-97701V)Discover more about diseases related to Nidogen-1/Entactin Antibody (NBP1-97701V).
| Pathways for Nidogen-1/Entactin Antibody (NBP1-97701V)View related products by pathway.
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PTMs for Nidogen-1/Entactin Antibody (NBP1-97701V)Learn more about PTMs related to Nidogen-1/Entactin Antibody (NBP1-97701V).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NID1 |
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Flow-Cytometry-NBP1-47386-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Fibulin 2 Antibody [NBP1-88115] - Staining of human cell line BJ shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/images2/Fibulin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88115-img0004.jpg)
![Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Staining of human urinary bladder shows strong positivity in connective tissue.](http://images.novusbio.com/images2/Fibulin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88115-img0013.jpg)
![Immunohistochemistry-Paraffin: Fibulin 2 Antibody [NBP1-88115] - Analysis in human testis and liver tissues. Corresponding FBLN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Fibulin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88115-img0009.jpg)
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/images2/Tenascin-C-Antibody-(4C8MS)-Flow-(Intracellular)-NB110-68136-img0010.jpg)
![Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Tenascin-C-Antibody-(4C8MS)-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Tenascin-C-Antibody-(4C8MS)-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.](http://images.novusbio.com/images2/Glypican-3-Antibody-(9C2)-Western-Blot-NBP2-37483-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/Glypican-3-Antibody-(9C2)-Immunocytochemistry-Immunofluorescence-NBP2-37483-img0004.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of breast cancer tissues using GPC3 mouse mAb with DAB staining.](http://images.novusbio.com/images2/Glypican-3-Antibody-(9C2)-Immunohistochemistry-NBP2-37483-img0007.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
