Nicotinic Acetylcholine Receptor beta 4 Antibody

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 4 Antibody [NBP1-20901] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Nicotinic Acetylcholine Receptor beta 4 Antibody Summary

• Description: Novus Biologicals Goat Nicotinic Acetylcholine Receptor beta 4 Antibody (NBP1-20901) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Nicotinic Acetylcholine Receptor beta 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DYRLTWNSSRYEGVN corresponding to internal region according to NP_000741.1.
• Epitope: C terminus
• Specificity: The immunizing peptide represents part of the first exrracellular domain (aa22-236).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHRNB4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.2 ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Uterus.

Reactivity Notes

Expected from sequence similarity: Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nicotinic Acetylcholine Receptor beta 4 Antibody

• cholinergic receptor, nicotinic, beta 4
• cholinergic receptor, nicotinic, beta polypeptide 4
• CHRNB4
• NAChRB4
• neuronal acetylcholine receptor subunit beta-4
• neuronal nicotinic receptor beta 4 subunit
• Nicotinic Acetylcholine R beta 4

Background

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero) pentamers composed of homologous subunits. After binding acetylcholine, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nicotinic Acetylcholine Receptor beta 4 Antibody (NBP1-20901)(1)

Publication using NBP1-20901

• Liang Y, Salas R, Marubio L et al. Functional polymorphisms in the human beta4 subunit of nicotinic acetylcholine receptors. Neurogenetics 2005-02-01 [PMID: 15742216]

FAQs for Nicotinic Acetylcholine Receptor beta 4 Antibody (NBP1-20901). (Showing 1 - 1 of 1 FAQ).

1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNB4
• Entrez:
  • 1143(Human)
• Uniprot:
  • NP_000741.1(Human)