Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) [NBP2-61667] - Analysis using CHRNA2 mAb against HEK293 (1) and CHRNA2 (AA: extra 27-264)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) [NBP2-61667] - Analysis using CHRNA2 mAb against human CHRNA2 (AA: extra 27-264) recombinant protein. (Expected MW is 53.3 kDa)

Western Blot: Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) [NBP2-61667] - Analysis using CHRNA2 mouse mAb against SK-N-SH (1), SH-SY5Y (2), membrane protein of C6 (3), and SW480 (4) cell lysate.

ELISA: Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) [NBP2-61667] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 7G12H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 2/CHRNA2 (AA: extra 27-264) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRNA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (7G12H9) - BSA Free

• cholinergic receptor, nicotinic, alpha 2 (neuronal)
• cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal)
• CHRNA2
• neuronal acetylcholine receptor subunit alpha-2
• Nicotinic Acetylcholine R alpha 2
• Nicotinic Acetylcholine Ra2

Background

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Nicotinic Acetylcholine R alpha 2/CHRNA2 Antibody (NBP2-61667). (Showing 1 - 1 of 1 FAQ).

1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNA2