Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8)

Images

 
Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin ...read more
Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.
Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).
Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
5B6G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) Summary

Immunogen
Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHRNA6
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:1000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8)

  • CHNRA6
  • cholinergic receptor, nicotinic, alpha 6
  • cholinergic receptor, nicotinic, alpha polypeptide 6
  • CHRNA6
  • neuronal acetylcholine receptor subunit alpha-6
  • Nicotinic Acetylcholine R alpha 6
  • Nicotinic Acetylcholine Ra6

Background

This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CHRNA6
Entrez