Nicotinic Acetylcholine Receptor beta 2 Antibody

Images

 
Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467] - Analysis of Nicotinic Acetylcholine Receptor beta 2 in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-28467 at 1ug/ml. ...read more
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Nicotinic Acetylcholine Receptor beta 2 Antibody Summary

Immunogen
Peptide with sequence QPRHHCARQRLR corresponding to internal region according to NP_000739.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHRNB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 48 kDa band observed in human brain (cerebellum, cerebral cortex) lysates (calculated MW of 57.0 kDa band according to NP_000739.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-28467.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nicotinic Acetylcholine Receptor beta 2 Antibody

  • cholinergic receptor, nicotinic, beta 2 (neuronal)
  • cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
  • EFNL3
  • nAChRB2
  • neuronal acetylcholine receptor subunit beta-2
  • neuronal nicotinic acetylcholine receptor beta 2

Background

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nicotinic Acetylcholine Receptor beta 2 Antibody (NBP1-28467)(1)

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WB Video Protocol

FAQs for Nicotinic Acetylcholine Receptor beta 2 Antibody (NBP1-28467). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
    • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

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Bioinformatics

Gene Symbol CHRNB2
Entrez
Uniprot