Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody [NBP2-93514] - Analysis of extracts of various cell lines, using Nicotinic Acetylcholine R alpha 1/CHRNA1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Immunohistochemistry: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody - BSA Free [NBP2-93514] - Immunohistochemistry analysis of Nicotinic Acetylcholine R alpha 1/CHRNA1 in paraffin-embedded Human skeletal muscle tissue using Nicotinic Acetylcholine R alpha 1/CHRNA1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody - BSA Free [NBP2-93514] - Immunofluorescence analysis of paraffin-embedded Mouse heart using Nicotinic Acetylcholine R alpha 1/CHRNA1 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 105-220 of human CHRNA1 (NP_000070.1). IWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRNA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody - BSA Free

• acetylcholine receptor subunit alpha
• ACHRA
• ACHRD
• CHNRA
• cholinergic receptor, nicotinic, alpha 1 (muscle)
• CHRNA
• CHRNA1
• CMS2A
• FCCMS
• nAChR alpha 1
• nAchRa1
• Nicotinic Acetylcholine R alpha 1
• Nicotinic Acetylcholine Ra1
• nicotinic acetylcholine receptor alpha subunit
• nicotinic cholinergic receptor alpha 1
• nicotinic, alpha polypeptide 1 (muscle)
• SCCMS

Background

The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha isoforms and 1 each of beta, gamma, and delta subunits.2 This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (NBP2-93514). (Showing 1 - 1 of 1 FAQ).

1. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNA1