NGL-3/LRRC4B/LRCH4B Antibody (702021)

NGL-3/LRRC4B was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human NGL-3/ LRRC4B Monoclonal Antibody (Catalog # MAB6919) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 702021
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NGL-3/LRRC4B/LRCH4B Antibody (702021) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human NGL-3/LRRC4B
  Ala47-Lys576
  Accession # Q9NT99
• Specificity: Detects human NGL-3/LRRC4B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LRRC3, 4, or 32 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRRC4B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGL-3/LRRC4B/LRCH4B Antibody (702021)

• DKFZp761A179
• HSM
• Leucine Rich Repeat Containing 4B
• Leucine-Rich Repeat-Containing Protein 4B
• Leucine-Rich Repeats And Immunoglobulin-Like Domains 4
• LRIG4
• LRRC4B
• Netrin-G3 Ligand
• NGL3
• NGL-3

Background

NGL-3 (netrin-G ligand-3), also called LRRC4B (Leu-rich repeat/LRR-containing glycoprotein 4B), is a 72 kDa (predicted) post-synaptic adhesion molecule of the LRR protein family. Mature human LRRC4B is a 678 amino acid (aa) type I transmembrane protein with a 541 aa extracellular region (aa 36-576) containing nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. Over the region used as an immunogen, human LRRC4B shares 97% aa sequence identity with mouse and rat LRRC4B. LRRC4B also shares ~55% aa identity with LRRC4C/NGL-1 and LRRC4/NGL-2, but each recognizes different ligands. LRRC4B recognition of the receptor tyrosine phosphatases LAR/PTPRF, PTPR-d and PTPR-s induces excitatory synapse formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC4B
• Uniprot:
  • Q9NT99()