NGFI-B alpha/Nur77/NR4A1 Antibody

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394] - (2ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394] - (5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NGFI-B alpha/Nur77/NR4A1 Antibody Summary

• Description: Novus Biologicals Goat NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-55394) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-NGFI-B alpha/Nur77/NR4A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GRLPSKPKQPPDAS corresponding to internal region according to NP_775180.1.
• Epitope: GRLPSKPKQPPDAS
• Specificity: This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NR4A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:1000
• Application Notes: WB: Approx. 60 kDa band observed in human thyroid gland lysates (calculated MW of 64.5 kDa band according to NP_002126.2 and NP_775180.1).
• Control:

  Human Thyroid Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody

• Early response protein NAK1
• GFRP1ST-59
• growth factor-inducible nuclear protein N10
• HMR
• HMRNP10
• hormone receptor
• MGC9485
• N10
• NAK1
• NAK-1
• NGFIB alpha
• NGFI-B alpha
• NGFIB
• NR4A1
• Nuclear hormone receptor NUR/77
• nuclear receptor subfamily 4 group A member 1
• nuclear receptor subfamily 4, group A, member 1
• Nur77
• Orphan nuclear receptor HMR
• Orphan nuclear receptor TR3
• steroid receptor TR3
• Testicular receptor 3
• TR3 orphan receptor
• TR3

Background

Nak1, a NR4 Nerve Growth Factor-Like receptor, has been shown to affect the hypothalamic-pituitary axis, neuronal differentiation, neuroendocrine regulation of adrenocortical function, and T-cell apoptosis. Nak1 also is known as nuclear receptor Tr3 and is similar to mouse Nur77 and rat NGFI-B. It is encoded by one of the immediate early genes (IEG) in response to nerve growth factor and other serum growth factors. Nak1 can bind to DNA as a monomer, homodimer, or heterodimer (with retinoid X receptors). Nak1 has been shown to inhibit vascular lesion formation and could serve as a novel target in the treatment of vascular diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-55394)(1)

Publication using NB100-55394

• Kiss I, Oskolas H, Toth R et al. Adenosine A(2A )receptor-mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77. Eur J Immunol 2006-06-01 [PMID: 16673448]

Bioinformatics

• Gene Symbol: NR4A1
• Entrez:
  • 3164(Human)
  • 15370(Mouse)
  • 79240(Rat)
• Uniprot:
  • NP_002126.2(Human)