NFYB Antibody

Immunohistochemistry-Paraffin: NFYB Antibody [NBP1-02912] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Product Discontinued

NFYB Antibody Summary

• Immunogen: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein, conjugated with KLH.
• Localization: Nucleus
• Specificity: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFYB
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:25000
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500 - 1:3000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Delipidated and defibrinated serum
• Preservative: 0.01% Sodium Azide
• Concentration: 90 mg/ml
• Purity: Delipidation and Defibrination

Alternate Names for NFYB Antibody

• CAAT box DNA-binding protein subunit B
• CBF-A
• HAP3CBF-B
• NF-YBCCAAT-binding transcription factor subunit A
• Nuclear transcription factor Y subunit B
• nuclear transcription factor Y subunit beta
• nuclear transcription factor Y, beta
• Transcription factor NF-Y, B subunit

Background

The protein encoded by the NFYB gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFYB
• Entrez:
  • 4801(Human)
• Uniprot:
  • P25208(Human)