Human NFkB1/NFkB p105 ELISA Kit (Colorimetric)

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Standard Curve Reference.
Samples were spiked with high concentrations of Human NFkB1/NFkB p105, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.1 ng/mL

Order Details

Human NFkB1/NFkB p105 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 3 h 30 min
Specificity
This kit recognizes Human NFkB1/NFkB p105 in samples. No significant cross-reactivity or interference between Human NFkB1/NFkB p105 and analogues was observed
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.1 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NFKB1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human NFkB1/NFkB p105 ELISA Kit (Colorimetric)

  • DNA-binding factor KBF1
  • EBP-1
  • NF-kappa-B
  • NFkB1
  • NF-kB1
  • NFKB-p105
  • Nuclear factor NF-kappa-B p105 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
  • p105

Background

Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for NFkB1/NFkB p105 ELISA Kit (NBP3-49852). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol NFKB1