Human NFkB1/NFkB p105 ELISA Kit (Colorimetric) Summary
| Description |
Assay Length: 3 h 30 min |
| Specificity |
This kit recognizes Human NFkB1/NFkB p105 in samples. No significant cross-reactivity or interference between Human NFkB1/NFkB p105 and analogues was observed |
| Standard Curve Range |
0.16 - 10 ng/mL |
| Sensitivity |
0.1 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10 |
| Intra-Assay |
CV% < 10 |
| Spike Recovery |
80 - 120% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
NFKB1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Certificate of Analysis
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Description
- Reference Standard & Sample Diluent
- Reference Standard
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human NFkB1/NFkB p105 ELISA Kit (Colorimetric)
Background
Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for NFkB1/NFkB p105 ELISA Kit (NBP3-49852). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.