NFkB p50 (NLS) Inhibitor Peptide Set

Images

 
Functional (Inhibition): NFkB p105/p50 Inhibitor Peptide Set [NBP2-29323] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, XpSpecies Glossary
Applications Func-Inh
Concentration
LYOPH

Order Details

NFkB p50 (NLS) Inhibitor Peptide Set Summary

Immunogen
Functions as a p50 decoy by blocking the intracellular recognition mechanism of p50 NLS.
Specificity
The p50 NLS inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.

The control peptide consists of only the PTD sequence
Preparation
Method
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions.
Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. p50 (NLS)
Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKVQRKRQKLM
Add 57 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
Content
NF-kB p50 (NLS) Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKVQRKRQKLM (p50 NLS sequence: VQRKRQKLM). Molecular weight: 3529. Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
Gene
NFKB1

Applications/Dilutions

Application Notes
Inhibition of NF-kB p50(NLS) activity. The inhibitor peptide is designed to block p50 nuclear translocation. Optimal peptide concentrations and incubation times vary between model systems and should be determined empirically by users. A 100 uM final concentration may be a useful starting point. Please refer to Lin et al (1995) for additional information about how the inhibitor peptide has been used to block p50 nuclear translocation. Use in Functional inhibition reported in scientific literature (PMID 24384074)
Publications
Read Publications using
NBP2-29323 in the following applications:

Reactivity Notes

Broad: Peptide sequence is 100% conserved across multiple species. Reactivity includes human, mouse, rat, dog, cow, chicken, and xenopus.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration
LYOPH
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Notes

The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)].The control peptide consists of only the PTD sequence.

Background

The NLS sequence is required for nuclear translocation of NF-kB p50. In stimulated cells, NF-kB is in the cytoplasm in an inactive form and the NLS is masked. The degradation of IkBalpha during NF-kB signaling unmasks the NLS of NF-kB subunits and NF-kB is translocated to the nucleus. The p50 inhibitory peptide contains the p50 NLS sequence (VQRKRQKLM) and blocks the intracellular recognition mechanism for the NLS present on p50. Consequently p50 nuclear translocation is blocked.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Publications for NFkB p50 (NLS) Inhibitor (NBP2-29323)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Func-INH.


Filter By Application
Func-INH
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP2-29323 Applications Species
Ishizuka EK, Filgueiras LR, Rios FJ et al. PAFR activation of NF-kB p65 or p105 precursor dictates pro- and anti-inflammatory responses during TLR activation in murine macrophages. Sci Rep. Aug 24 2016 [PMID: 27554194] (Mouse) Mouse
Stassi Stefano, Cauda Valentina, Canavese Giancarlo, Pirri Candido Fabrizio. Flexible tactile sensing based on piezoresistive composites: a review. Sensors (Basel). 2014 [PMID: 24638126] (Human) Human
Bakhru P, Sirisaengtaksin N, Soudani E et al. BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9. Cell Immunol 2014 Jan [PMID: 24384074] (Func-INH, Mouse) Func-INH Mouse
Park Jang-Yeon, Moeller Steen, Goerke Ute et al. Short echo-time 3D radial gradient-echo MRI using concurrent dephasing and excitation. Magn Reson Med. 2012 Feb [PMID: 21702064] (Human) Human
Ababneh Sufyan Y, Prescott Jeff W, Gurcan Metin N. Automatic graph-cut based segmentation of bones from knee magnetic resonance images for osteoarthritis research. Med Image Anal. 2011 Aug [PMID: 21474362] (Human) Human
Branski RC, Zhou H, Sandulache VC et al. Cyclooxygenase-2 signaling in vocal fold fibroblasts. Laryngoscope. 2010 Sep [PMID: 20717945]

Details:
Product cited: 1. NF-kB p50 (NLS) Inhibitory Peptide Set (IMG-2004): HVOX human vocal fold fibroblasts cell line, Fig 3A, B. Readout assays measuring ratio of nuclear to cytoplasmic p50 (Fig 3A) and COX-2 transcription (Fig 3B) were used to measure the ef
Fang X, Neyrinck AP, Matthay MA, Lee JW. Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1. J Biol Chem. 2010 Aug 20 [PMID: 20554518]

Details:
Product cited: 1. NF-kB p50 (NLS) Inhibitory Peptide Set (IMG-2004): Primary human alveolar epithelial Type II Cells, Fig 9D. An epithelial permeability to protein assay was used as a readout to measure the effects of the p50 peptide. The p50 peptide was
Mohamed JS, Lopez MA, Cox GA, Boriek AM. Anisotropic regulation of Ankrd2 gene expression in skeletal muscle by mechanical stretch. FASEB J. 2010 Sep [PMID: 20442316]

Details:
1. NF-kB p50 (NLS) Inhibitory Peptide Set (IMG-2004): Fig 4AB.
Buchau AS, MacLeod DT, Morizane S et al. Bcl-3 acts as an innate immune modulator by controlling antimicrobial responses in keratinocytes. J Invest Dermatol. 2009 Sep [PMID: 19282837]
Haker Steven, Wells William M 3rd, Warfield Simon K et al. Combining classifiers using their receiver operating characteristics and maximum likelihood estimation. Med Image Comput Comput Assist Interv. 2005 [PMID: 16685884]

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Product General Protocols

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FAQs for NFkB p50 (NLS) Inhibitor (NBP2-29323). (Showing 1 - 1 of 1 FAQs).

  1. Please let us know the buffer composition prior to lyophilization. Is it lyophilized from H2O?
    • Thank you for your inquiry about NBP2-20323, the NFkB p105/p50 Inhibitor Peptide Set. This product is lyophilized from a PBS buffer.

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Bioinformatics

Gene Symbol NFKB1