NFIX Antibody (HL1269) - Azide and BSA Free

Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:250. Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] diluted at 1:500.

Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Mu, Rt, Hu
• Applications: ICC/IF, IHC
• Clone: HL1269
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NFIX Antibody (HL1269) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit NFIX Antibody (HL1269) - Azide and BSA Free (NBP3-25576) is a recombinant monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.
• Source: HEK293
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFIX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for NFIX Antibody (HL1269) - Azide and BSA Free

• CTF
• NF1ACCAAT-box-binding transcription factor
• NF1-X
• NF-I/X
• NFI-X
• nuclear factor 1 X-type
• Nuclear factor 1/X
• nuclear factor I/X (CCAAT-binding transcription factor)
• Nuclear factor I/X
• TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIX