NFATC4 Antibody

Immunohistochemistry-Paraffin: NFATC4 Antibody [NB100-1082] - 3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NFATC4 Antibody Summary

• Immunogen: Peptide with sequence C-SGFPAPPGEEPPA corresponding to C-Terminus according to NP_004545.2, NP_001185894.1, NP_001185895.1, NP_001185896.1.
• Specificity: This antibody is expected to recognise isoform 2, 3, 4 and 5 (NP_004545.2; NP_001185894.1; NP_001185895.1; NP_001185896.1 respectively).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NFATC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: No signal obtained yet but low background observed in MOLT-4, Jurkat, A431, HeLa, human brain and human heart lysates at upto 2ug/ml. IHC-P: Human placenta shows weak nuclear staining in trophoblasts.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NFATC4 Antibody

• NF-AT3
• NFAT3nuclear factor of activated T-cells, cytoplasmic 4
• NFATc4
• NF-ATc4
• nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
• T cell transcription factor NFAT3
• T-cell transcription factor NFAT3

Background

The protein is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFATC4 Antibody (NB100-1082)(3)

Publications using NB100-1082

• Stafylis C, Bristow CC, Natoli LJ et al. Field evaluation of a dual rapid Human Immunodeficiency Virus and treponemal syphilis rapid test in community-based clinics in Los Angeles and New York Diagnostic microbiology and infectious disease 2020-11-05 [PMID: 30704779]
• Trost MJ, Cowell M, Cannon J et al. Risk factors for overnight respiratory events following sedation for magnetic resonance imaging in children with sleep apnea Sleep & breathing = Schlaf & Atmung 2016-09-28 [PMID: 27683261]
• Hoey T, Sun YL, Williamson K, Xu X. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins. Immunity 1995-05-01 [PMID: 7749981]

Bioinformatics

• Gene Symbol: NFATC4
• Entrez:
  • 4776(Human)
• Uniprot:
  • NP_004545(Human)