Neuropilin-2 Antibody (96009R) [Unconjugated]

Western blot shows lysates of C6 rat glioma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

C6 Rat glial tumor cell line was stained with Mouse Anti-Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567R, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Simple Western lane view shows lysates of C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 138 kDa (as indicated) using 50 µg/mL of Mouse Anti-Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567R) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Rt
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clone: 96009R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Neuropilin-2 Antibody (96009R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
  Gln23-Asp857 (Val809-Asp825 del)
  Accession # O35276
• Specificity: Detects rat Neuropilin-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-2 Antibody (96009R) [Unconjugated]

• MGC126574
• neuropilin 2
• Neuropilin2
• Neuropilin-2
• neuropilin-2a(22)
• neuropilin-2b(0)
• NP2
• NPN 2
• NPN2
• PRO2714
• receptor for VEGF165 and semaphorins class3
• Vascular endothelial cell growth factor 165 receptor 2
• vascular endothelial growth factor-165 receptor 2
• VEGF1265R2
• VEGF165R2neuropilin-2a(17)

Background

Neuropilin-1 (Npn-1, previously known as neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn-1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF 165 . Npn-1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
2. Neufeld, G. et al. (1999) FASEB J. 13:9.
3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O35276()