Neuropeptide FF R1/NPFFR1 Antibody

Western Blot: NPFF1 Receptor Antibody [NB100-93424] - Analysis of NPFF1 Receptor in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer) using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Neuropeptide FF R1/NPFFR1 Antibody Summary

• Immunogen: Peptide with sequence C-RPSGSHKEAYSERP corresponding to internal region according to NP_071429.1.
• Epitope: C-RPSGSHKEAYSERP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPFFR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human brain (cerebellum and cerebral cortex) lysates (calculated MW of 47.8 kDa band according to NP_071429.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neuropeptide FF R1/NPFFR1 Antibody

• FLJ10751
• G protein-coupled receptor 147
• GPR147
• neuropeptide FF 1
• Neuropeptide FF R1
• neuropeptide FF receptor 1
• Neuropeptide FFR1
• NPFF1
• NPFF1G-protein coupled receptor 147
• NPFF1R1
• NPFFR1
• OT7T022
• RFamide-related peptide receptor OT7T022

Background

The central nervous system neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance as well as other physiological functions. Two G protein-coupled receptors, NPFF1 and NPff2, were isolated from the human central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuropeptide FF R1/NPFFR1 Antibody (NB100-93424)(1)

Publication using NB100-93424

• Gouarderes C, Mazarguil H, Mollereau C et al. Functional differences between NPFF1 and NPFF2 receptor coupling: high intrinsic activities of RFamide-related peptides on stimulation of [35S]GTPgammaS binding. Neuropharmacology 2007-02-01 [PMID: 17011599]

Bioinformatics

• Gene Symbol: NPFFR1
• Entrez:
  • 64106(Human)
• Uniprot:
  • NP_071429.1(Human)