GPR74/NPFFR2 Antibody - BSA Free

Western Blot: NPFFR2 Antibody [NB300-169] - Analysis of human NPFF2 in brain lysate using this antibody at a dilution of 1:500

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GPR74/NPFFR2 Antibody - BSA Free Summary

• Immunogen: N terminal sequence MNEKWDTNSSENWHPI and the C terminal sequence ELVMEELKETTNSSEI of the human NPFF2 protein.
• Localization: Cell membrane
• Specificity: Reacts with the N terminal sequence MNEKWDTNSSENWHPI and the C terminal sequence ELVMEELKETTNSSEI of the human NPFF2 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPFFR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:500 -1:1000
• Application Notes: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR74/NPFFR2 Antibody - BSA Free

• GPR74
• GPR74G protein-coupled receptor 74
• G-protein coupled receptor 74
• neuropeptide FF 2
• neuropeptide FF receptor 2
• Neuropeptide G-protein coupled receptor
• NPFF2
• NPFF2G-protein coupled receptor HLWAR77
• NPFFR2
• NPGPR
• NPGPRHLWAR77

Background

The central nervous system neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance as well as other physiological functions. Two G protein-coupled receptors, NPFF1 and NPFF2, were isolated from the human central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR74/NPFFR2 Antibody (NB300-169)(3)

Publications using NB300-169

• Shin Y, Jung W, Kim MY et al. NPFFR2 Contributes to the Malignancy of Hepatocellular Carcinoma Development by Activating RhoA/YAP Signaling Cancers 2022-11-27 [PMID: 36497331] (ICC/IF, Human)
• Torz, L;Niss, K;Lundh, S;Rekling, JC;Quintana, CD;Frazier, SED;Mercer, AJ;Cornea, A;Bertelsen, CV;Gerstenberg, MK;Hansen, AMK;Guldbrandt, M;Lykkesfeldt, J;John, LM;Villaescusa, JC;Petersen, N; NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model International journal of molecular sciences [PMID: 35328681] (Human)
• Sun Y, Zhang X, He N et al. Neuropeptide FF activates ERK and NF kappa B signal pathways in differentiated SH-SY5Y cells Peptides 2012-01-01 [PMID: 22981806] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: NPFFR2
• Entrez:
  • 10886(Human)