Neuroligin 2/NLGN2 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Neuroligin 2/NLGN2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Neuroligin 2/NLGN2
  Gln15-Ser660
  Accession # EAW90197
• Specificity: Detects human and rat Neuroligin 2/NLGN2 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroligin 2/NLGN2 Antibody [Alexa Fluor® 488]

• EC 3.1.1
• EC 3.1.1.1
• KIAA1366neuroligin-2
• Neuroligin 2
• NLGN2

Background

Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8 - 10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11‑15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2‑dependent GABAergic contacts (7, 15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.