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Neurogenin-3 Antibody (4B7)

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Immunocytochemistry/Immunofluorescence: Neurogenin-3 Antibody (4B7) [H00050674-M01] - Analysis of monoclonal antibody to NEUROG3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (4B7) [H00050674-M01] - Analysis of monoclonal antibody to NEUROG3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: Neurogenin-3 Antibody (4B7) [H00050674-M01] - Analysis of NEUROG3 transfected lysate using anti-NEUROG3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEUROG3 MaxPab rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
4B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Neurogenin-3 Antibody (4B7) Summary

Immunogen
NEUROG3 (NP_066279, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag.MTPQPSGAPTVQVTRETERSFPRASEDEVTCPTSAPPSPTRTRGNCAEAEEGGCRGAPRKLRARRGGRSRPKSELALSKQRRSRRKKANDRERNRMHNLN
Specificity
NEUROG3 - neurogenin 3
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEUROG3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Neurogenin-3 Antibody (4B7)

  • Atoh5
  • BHLHA7
  • bHLHa7NGN-3
  • Class A basic helix-loop-helix protein 7
  • Math4B
  • NEUROG3
  • neurogenin 3
  • Neurogenin3
  • Neurogenin-3
  • NGN3
  • Protein atonal homolog 5
  • Relax

Background

Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm (Gradwohl et al., 2000 [PubMed 10677506]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human stomach shows strong cytoplasmic(granular pattern) and nuclear positivity in glandular cells.
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Pax4 Antibody
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(196908)
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KLRG1 Antibody
(697213)
MAB70291
Western blot shows lysates of NK‑92 human natural killer lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for KLRG1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Neurogenin-3
Antibody (4B7)
H00050674-M01
Neurogenin-3 Antibody (4B7)
Species: Hu
Applications: WB, ELISA, ICC/IF, IP

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Bioinformatics

Gene Symbol NEUROG3
Entrez
OMIM
Uniprot