Neuregulin-1/NRG1 Overexpression Lysate (Native)

Images

 
Western Blot: NRG1 Overexpression Lysate (Adult Normal) [NBL1-13790] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRG1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Neuregulin-1/NRG1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC220134 and based on accession number NM_013956. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens neuregulin 1 (NRG1), transcript variant HRG-beta1, mRNA.
Gene
NRG1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Neuregulin-1/NRG1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Neuregulin-1/NRG1 Overexpression Lysate (Native)

  • ARIA
  • GGF2
  • GGFglial growth factor
  • Heregulin-1
  • HGL
  • HGLneu differentiation factor
  • HRG
  • HRG1
  • HRG1-alpha
  • HRG1-beta 1
  • HRGAneuregulin 1 type IV beta 1a
  • MST131
  • MSTP131
  • NDFheregulin, alpha (45kD, ERBB2 p185-activator)
  • neuregulin 1 type IV beta 3
  • neuregulin 1
  • Neuregulin1
  • Neuregulin-1
  • NRG1
  • pro-neuregulin-1, membrane-bound isoform
  • pro-NRG1
  • sensory and motor neuron derived factor
  • SMDF

Background

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq]. Transcript Variant: This variant (HRG-beta1), along with HRG-alpha, beta2, and beta3 variants, was identified in various normal tissues and cancer cell lines. The protein product encoded by this variant is distinct from that of HRG-alpha in the region of amino acids 213-239. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NRG1
Entrez