Neuregulin-1/NRG1 Antibody (147724) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB
• Clone: 147724
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

Neuregulin-1/NRG1 Antibody (147724) [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human Neuregulin‑1/NRG1
  Ser2-Lys246
  Accession # AAA58639
• Specificity: Detects human Neuregulin‑1/NRG1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with the EGF‑like domains of recombinant human (rh) NRG1-beta 1 (aa 176‑246) or rhNRG1-alpha (aa 177‑241). Therefore, this antibody recognizes an epitope located within aa 2‑176 segment N‑terminal to the EGF‑like domain. This segment is present in all NRG1‑ alpha , beta and gamma isoforms and in NDF43 and glial growth factor (GGF). It is absent from the NRG1 gene products GGF2 and SMDF.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRG1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Neuregulin-1/NRG1 Antibody (147724) [Janelia Fluor® 525]

• ARIA
• GGF2
• GGFglial growth factor
• Heregulin-1
• HGL
• HGLneu differentiation factor
• HRG
• HRG1
• HRG1-alpha
• HRG1-beta 1
• HRGAneuregulin 1 type IV beta 1a
• MST131
• MSTP131
• NDFheregulin, alpha (45kD, ERBB2 p185-activator)
• neuregulin 1 type IV beta 3
• neuregulin 1
• Neuregulin1
• Neuregulin-1
• NRG1
• pro-neuregulin-1, membrane-bound isoform
• pro-NRG1
• sensory and motor neuron derived factor
• SMDF

Background

Neuregulin 1 (NRG1, isoform HRG-beta2) belongs to the neuregulin/heregulin cytokine family consisting of variants of the four genes NRG1, NRG2, NRG3 and NRG4. More than 15 transmembrane and secreted protein isoforms exist for NRG1, they are generated by alternative promoter usage and mRNA splicing. The different isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. The EGF-like domain interacts with ErbB3 and ErbB4 tyrosine kinase receptors, resulting in tyrosine phosphorylation and NRG1 signaling. NRG proteins show distinct spatial and temporal expression patterns. These proteins are important for the development of the nervous system and the heart and in the regulation of neurotransmission.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRG1