NEU2 Antibody (OTI4F4) - Azide and BSA Free

Western Blot: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2.

Immunocytochemistry/Immunofluorescence: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2.

Flow Cytometry: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944] - Analysis of Hela cells, using anti-NEU2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI4F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NEU2 Antibody (OTI4F4) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human NEU2 (NP_005374) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEU2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 42.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for NEU2 Antibody (OTI4F4) - Azide and BSA Free

• Cytosolic sialidase
• EC 3.2.1.18
• N-acetyl-alpha-neuraminidase 2
• NEU2
• neuraminidase 2
• SIAL2MGC129579
• sialidase 2 (cytosolic sialidase)
• sialidase-2

Background

NEU2 belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEU2