NELL2 Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

NELL2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse NELL2
  Leu25-Leu819
  Accession # NP_058023
• Specificity: Detects mouse NELL2 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) NELL2 and less than 3% cross-reactivity with rhNELL1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NELL2 Antibody [Alexa Fluor® 488]

• Mel91
• nel (chicken)-like 2
• NELL2
• NEL-like 2 (chicken)
• NEL-like protein 2
• Nel-related protein 2
• NRP2
• NRP2neural epidermal growth factor-like 2
• protein kinase C-binding protein NELL2

Background

NELL2 (neural EGF-like like protein 2) is a secreted, 130-140 kDa glycoprotein member of the EGF-like domain containing family, Laminin G/N-TSP1/Pentraxin gene superfamily of molecules. It is expressed in both fetal and postnatal neurons, being found in pyramidal and hypothalamic glutamatergic neurons, and in GABAergic cerebellar Purkinje cells. It has multiple functions, including the induction of GnRH release and the promotion of neuronal neurite extension and synapse formation. The mouse NELL2 precursor is 819 amino acids (aa) in length. It contains a 27 aa signal sequence plus a 792 aa mature region. The mature region possesses an N‑terminal TSP domain (aa 33-261), two VWFC domains (aa 275-399), six consecutive EGF-like domains (aa 400-640), and three C-terminal VWFC domains (aa 641‑816). Secreted NELL2 forms 400 kDa noncovalent homotrimers. There are four potential alternate splice forms. One utilizes an alternate start site at Met4, while a second shows a 16 aa substitution for aa 400-436. Based on rat, two other isoforms are likely to exist. A third isoform (cNELL2) should generate a 90 kDa phosphorylated cytosolic molecule that possesses a deletion of aa 22-64, while a fourth, 30 kDa isoform (NELL2-Tsp) should arise due to a premature truncation after Ala261. This last isoform is soluble and predicted to trimerize with full-length NELL2, yielding lower MW complexes. Over aa 24-819, mouse NELL2 shares 97% and 94% aa identity with rat and human NELL2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.