NELL1 Antibody

Western Blot: NELL1 Antibody [NBP2-19511] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NELL1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: NELL1 Antibody [NBP2-19511] - Human breast cancer, using NELL1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: NELL1 Antibody [NBP2-19511] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NELL1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Discontinued

NELL1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human NELL1. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NELL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for NELL1 Antibody

• FLJ45906
• IDH3GL
• nel (chicken)-like 1
• NELL1
• NEL-like 1 (chicken)
• NEL-like protein 1
• Nel-related protein 1
• neural epidermal growth factor-like 1
• NRP1
• protein kinase C-binding protein NELL1

Background

This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NELL1
• Uniprot:
  • Q92832()