NEK8 Antibody

Images

 
Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.
Western Blot: NEK8 Antibody [NBP1-82539] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NEK8 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLG
Specificity
Specificity of human, mouse, rat NEK8 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEK8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
NEK8 Protein (NBP1-82539PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NEK8 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • JCKNIMA-related kinase 12a
  • MGC138445
  • NEK12A
  • nephrocystin 9
  • Never in mitosis A-related kinase 8
  • NIMA (never in mitosis gene a)- related kinase 8
  • Nima-related protein kinase 12a
  • NimA-related protein kinase 8
  • NPHP9NIMA-family kinase NEK8
  • serine/threonine-protein kinase Nek8
  • serine/thrionine-protein kinase NEK8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NEK8 Antibody (NBP1-82539). (Showing 1 - 1 of 1 FAQ).

  1. The molecular size of NEK8 is 75kDa. If you look at the WB image on the web for NBP1-82539(NEK8 antibody), the band is located between 70-100kDa (Closer to 100kDa). In our WB testing, we also observed the band near 100kDa. Do you think that it is the band of NEK8?
    • The predicted size of NEK8 protein is 75 kDa. However, the actual observed size of the protein is dependent on the sample type. If the protein has undergone post-translational modifications, then the size of the protein band could be higher or lower than the predicted one. Without a positive control such as a purified protein or overexpressed lysate, it is very hard to confirm 100% whether the band is real or not. We have not specifically tested MCF7 cell line, however we suspect that the band observed by you is the post-translationally modified NEK8 protein running at higher than predicted molecular weight.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEK8