NEK8 Antibody - BSA Free

Immunohistochemistry-Paraffin: NEK8 Antibody [NBP1-82539] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NEK8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NEK8 Antibody - BSA Free (NBP1-82539) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  NEK8 Protein (NBP1-82539PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Mouse (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NEK8 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• JCKNIMA-related kinase 12a
• MGC138445
• NEK12A
• nephrocystin 9
• Never in mitosis A-related kinase 8
• NIMA (never in mitosis gene a)- related kinase 8
• Nima-related protein kinase 12a
• NimA-related protein kinase 8
• NPHP9NIMA-family kinase NEK8
• serine/threonine-protein kinase Nek8
• serine/thrionine-protein kinase NEK8

Background

NEK8 (NIMA-Related Kinase 8) belongs to the protein kinase superfamily. Mutations in NEK8 have been linked to nephronophthisis type 9. NEK8 is required for renal tubular integrity. NEK8 is known to have interactions with ABL1, FYN, ACVR1, BICD2 and CSN2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NEK8 Antibody (NBP1-82539). (Showing 1 - 1 of 1 FAQ).

1. The molecular size of NEK8 is 75kDa. If you look at the WB image on the web for NBP1-82539(NEK8 antibody), the band is located between 70-100kDa (Closer to 100kDa). In our WB testing, we also observed the band near 100kDa. Do you think that it is the band of NEK8?
   • The predicted size of NEK8 protein is 75 kDa. However, the actual observed size of the protein is dependent on the sample type. If the protein has undergone post-translational modifications, then the size of the protein band could be higher or lower than the predicted one. Without a positive control such as a purified protein or overexpressed lysate, it is very hard to confirm 100% whether the band is real or not. We have not specifically tested MCF7 cell line, however we suspect that the band observed by you is the post-translationally modified NEK8 protein running at higher than predicted molecular weight.

Bioinformatics

• Gene Symbol: NEK8