NEK7 Antibody

Western Blot: NEK7 Antibody [NBP1-68866] - Analysis of Mouse Lung (A), Mouse Testis (B), Rat Lung (C) and Rat Testis (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: NEK7 Antibody [NBP1-68866] - NBP1-68866 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NEK7 Antibody Summary

• Immunogen: Peptide with sequence CINPDPEKRPDVT corresponding to C-Terminus according to NP_598001.1.
• Epitope: CINPDPEKRPDVT
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEK7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 30-33 kDa band observed in human lung and testis lysates and in mouse and rat lung and testis lysates (calculated MW of 34.6 kDa band according to human NP_598001.1 and 34.5 kDa band according to mouse NP_067618.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK7 Antibody

• EC 2.7.11.1
• Never in mitosis A-related kinase 7
• NIMA (never in mitosis gene a)-related kinase 7
• NimA-related protein kinase 7
• serine/threonine-protein kinase Nek7

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEK7 Antibody (NBP1-68866)(1)

Publication using NBP1-68866

• Olson JE, Wang X, Pankratz VS et al. Centrosome-related genes, genetic variation, and risk of breast cancer. Breast Cancer Res Treat 2011-01-01 [PMID: 20508983]

Bioinformatics

• Gene Symbol: NEK7
• Entrez:
  • 140609(Human)
  • 59125(Mouse)
  • 360850(Rat)
• Uniprot:
  • NP_598001.1(Human)