NEK7 Antibody

Western Blot: NEK7 Antibody [NBP1-52115] - (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115] - 3.75ug/ml staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NEK7 Antibody Summary

• Description: Novus Biologicals Goat NEK7 Antibody (NBP1-52115) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-NEK7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NTLANFRIEKK corresponding to internal region according to NP_598001.1.
• Epitope: C-NTLANFRIEKK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEK7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.05-0.2ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.05 ug/ml
• Application Notes: Western blot: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Thyroid.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK7 Antibody

• EC 2.7.11.1
• Never in mitosis A-related kinase 7
• NIMA (never in mitosis gene a)-related kinase 7
• NimA-related protein kinase 7
• serine/threonine-protein kinase Nek7

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEK7 Antibody (NBP1-52115)(1)

Publication using NBP1-52115

• Olson JE, Wang X, Pankratz VS et al. Centrosome-related genes, genetic variation, and risk of breast cancer. Breast Cancer Res Treat 2011-01-01 [PMID: 20508983]

Bioinformatics

• Gene Symbol: NEK7
• Entrez:
  • 140609(Human)
  • 59125(Mouse)
  • 360850(Rat)
• Uniprot:
  • NP_598001.1(Human)