NEK7 Antibody

Images

 
Western Blot: NEK7 Antibody [NBP1-52115] - (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115] - 3.75ug/ml staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NEK7 Antibody Summary

Immunogen
Peptide with sequence C-NTLANFRIEKK corresponding to internal region according to NP_598001.1.
Epitope
C-NTLANFRIEKK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NEK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.05-0.2ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.05 ug/ml
Application Notes
Western blot: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Thyroid.
Publications
Read Publication using NBP1-52115.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NEK7 Antibody

  • EC 2.7.11.1
  • Never in mitosis A-related kinase 7
  • NIMA (never in mitosis gene a)-related kinase 7
  • NimA-related protein kinase 7
  • serine/threonine-protein kinase Nek7

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEK7 Antibody (NBP1-52115)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on NEK7

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Bioinformatics

Gene Symbol NEK7
Entrez
Uniprot