NEK6 Antibody

Immunohistochemistry-Paraffin: NEK6 Antibody [NLS7305] - Anti-NEK6 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK6 Antibody [NLS7305] - Analysis of anti-NEK6 antibody with human brain, Purkinje neurons at 13 ug/ml.

Immunohistochemistry-Paraffin: NEK6 Antibody [NLS7305] - Anti-NEK6 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: NEK6 Antibody [NLS7305] - Anti-NEK6 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Discontinued

NEK6 Antibody Summary

• Immunogen: Synthetic 18 amino acid peptide from near C-terminus of human NEK6.
• Specificity: Human NEK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NEK7 (61%).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 13 ug/ml
• Control:

  NEK6 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (89%), Orangutan (89%), Gibbon (89%), Marmoset (89%), Elephant (89%), Panda (89%), Canien (89%), Bat (89%), Rabbit (89%), Equine (89%), Xenopus (89%), Porcine (83%), Opossum (83%), Turkey (83%), Chicken (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK6 Antibody

• EC 2.7.11.1
• Never in mitosis A-related kinase 6
• NIMA (never in mitosis gene a)-related kinase 6
• NimA-related protein kinase 6
• Protein kinase SID6-1512
• putative serine-threonine protein kinase
• serine/threonine-protein kinase Nek6
• SID6-1512

Background

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK6
• Entrez:
  • 10783(Human)
• Uniprot:
  • Q9HC98(Human)