NEK2 Antibody (2F6) - Azide and BSA Free

Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.

Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 transfected lysate using anti-NEK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEK2 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: NEK2 Antibody (2F6) [H00004751-M01] - Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 2F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NEK2 Antibody (2F6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NEK2 (AAH43502, 331 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR
• Specificity: NEK2 (2F6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:2000
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEK2 Antibody (2F6) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• HSPK 21
• HsPK21
• NEK2
• NEK2AHsPK21
• Never in mitosis A-related kinase 2
• NIMA (never in mitosis gene a)-related kinase 2
• NimA-like protein kinase 1
• NimA-related protein kinase 2
• NLK1
• NLK1serine/threonine-protein kinase Nek2

Background

Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEK2 Antibody (H00004751-M01)(1)

Publication using H00004751-M01

• Li X, Liu Y, Zhou L et Al. Construction and Validation of a Mutation-Related Model in Papillary Renal Cell Carcinoma and Associated Immune Infiltration Kidney Blood Press Res 2024-06-11 [PMID: 38714185]

Bioinformatics

• Gene Symbol: NEK2
• Entrez:
  • 4751(Human)
• OMIM:
  • 604043(Human)
• Uniprot:
  • AAH43502(Human)