NEDP1/SENP8 Antibody (OTI3E4) - Azide and BSA Free

Western Blot: NEDP1/SENP8 Antibody (OTI3E4) - Azide and BSA Free [NBP2-72931] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP8.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NEDP1/SENP8 Antibody (OTI3E4) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human SENP8 (NP_660205) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SENP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for NEDP1/SENP8 Antibody (OTI3E4) - Azide and BSA Free

• DEN1
• DEN1deneddylase-1
• deneddylase 1
• Deneddylase-1
• EC 3.4.22.-
• HsT17512
• NEDD8-specific protease 1
• NEDP1
• NEDP1NEDD8 specific-protease cysteine 2
• Protease, cysteine 2
• protease, cysteine, 2 (NEDD8 specific)
• PRSC2
• SENP8
• Sentrin/SUMO-specific protease SENP8
• sentrin-specific protease 8
• SUMO sentrin specific protease family member 8
• SUMO/sentrin specific peptidase family member 8
• SUMO/sentrin specific protease family member 8

Background

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003 [PubMed 12759363]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SENP8