NEDL2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: NEDL2 Antibody [NBP1-92174] - Staining of human cell line A-431 shows localization to endoplasmic reticulum.

Immunohistochemistry-Paraffin: NEDL2 Antibody [NBP1-92174] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: NEDL2 Antibody [NBP1-92174] - Staining of human duodenum shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: NEDL2 Antibody [NBP1-92174] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: NEDL2 Antibody [NBP1-92174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in glial cells.

Analysis in human cerebral cortex and duodenum tissues using NBP1-92174 antibody. Corresponding HECW2 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NEDL2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NEDL2 Antibody - BSA Free (NBP1-92174) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HECW2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NEDL2 Protein (NBP1-92174PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NEDL2 Antibody - BSA Free

• C2 and WW domain-containing protein 2
• DKFZp686M17164
• EC 6.3.2
• EC 6.3.2.-
• HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
• NEDD4-like E3 ubiquitin-protein ligase 2

Background

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhanceactivation of transcription by TP73

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HECW2