Nectin-4/PVRL4 Antibody - BSA Free

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP2-20014] - MCF7 xenograft, using PVRL4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Nectin-4/PVRL4 Antibody [NBP2-20014] -HaCaT whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PVRL4 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Nectin-4/PVRL4 Antibody [NBP2-20014] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin-4/PVRL4 antibody [N1C1] (NBP2-20014) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt, Bv, Ca
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Nectin-4/PVRL4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Nectin-4/PVRL4. The exact sequence is proprietary.
• Predicted Species: Rat (94%), Canine (94%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NECTIN4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in (An M, Lee P, Choi S et al)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Nectin-4/PVRL4 Antibody - BSA Free

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Poliovirus receptor-like proteins (PVRLs), such as PVRL4, are adhesion receptors of the immunoglobulin superfamily and function in cell-cell adhesion (Reymond et al., 2001 [PubMed 11544254]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Nectin-4/PVRL4 Antibody (NBP2-20014)(1)

Publication using NBP2-20014

• An M, Lee P, Choi S et al. Erdosteine effects cell barriers causing decreased inflammation and increased pulmonary function in asthmatic mice exposed to nanoparticulate pollution European Journal of Inflammation 2023-12-01 (IHC-P, WB, Mouse)
  
  Details:
  WB Dilution: 1:1000; IHC-P Dilution 1:200

Bioinformatics

• Gene Symbol: NECTIN4
• Uniprot:
  • Q96NY8()