Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] - BSA Free

Staining of rat brain shows weak to moderate positivity in neuronal processes in the CA3 area of the hippocampus.

Staining of mouse brain shows weak to moderate positivity in neuronal processes in the CA3 area of the hippocampus.

Analysis in human cell lines U-251MG and MCF-7 using Nectin-3/PVRL3 antibody. Corresponding NECTIN3 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Analysis in human testis and skeletal muscle tissues using NBP3-43665 antibody. Corresponding NECTIN3 RNA-seq data are presented for the same tissues.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human duodenum shows strong membranous positivity in glandular cells.

Staining of human pancreas shows moderate membranous positivity in the islets of Langerhans.

Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL15366
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] - BSA Free Summary

• Description: Novus Biologicals Mouse Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] - BSA Free (NBP3-43665) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9NQS3, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NECTIN3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Nectin-3/PVRL3 Antibody (CL15366) [Unconjugated] - BSA Free

• CD113 antigen
• CD113
• CDw113FLJ90624
• DKFZp566B0846
• nectin 3
• Nectin3
• Nectin-3
• poliovirus receptor-related 3
• poliovirus receptor-related protein 3
• PPR3
• PRR3CD113
• PVRL3
• PVRR3

Background

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECTIN3