NDUFS2 Antibody

Western Blot: NDUFS2 Antibody [NBP1-52061] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Rb
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDUFS2 Antibody Summary

• Description: Novus Biologicals Goat NDUFS2 Antibody (NBP1-52061) is a polyclonal antibody validated for use in WB and ELISA. Anti-NDUFS2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SRGVRQWQPDVE corresponding to internal region according to NP_001159631.1.
• Epitope: C-SRGVRQWQPDVE
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004541.1; NP_001159631.1).
• Predicted Species: Porcine (100%), Bovine (100%), Rabbit (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDUFS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human, mouse and rat heart lysates (calculated MW of 51.9 kDa band according to NP_001159631.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS2 Antibody

• CI-49
• CI-49kD
• complex 1, mitochondrial respiratory chain, 49-KD subunit
• complex I 49kDa subunit
• Complex I-49kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 2 (49kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
• NADH-ubiquinone oxidoreductase 49 kDa subunit
• NADH-ubiquinone oxidoreductase NDUFS2 subunit

Background

Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS2 Antibody (NBP1-52061)(1)

Publication using NBP1-52061

• Saada A, Vogel RO, Hoefs SJ et al. Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease. Am J Hum Genet 2009-06-01 [PMID: 19463981]

Bioinformatics

• Gene Symbol: NDUFS2
• Entrez:
  • 4720(Human)
  • 226646(Mouse)
  • 289218(Rat)
• Uniprot:
  • NP_004541.1(Human)