NDUFB9 Antibody (OTI13H11) - Azide and BSA Free

Western Blot: NDUFB9 Antibody (OTI13H11) - Azide and BSA Free [NBP2-72919] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9.

Flow Cytometry: NDUFB9 Antibody (OTI13H11) - Azide and BSA Free [NBP2-72919] - Analysis of Jurkat cells, using NDUFB9 antibody,(Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: NDUFB9 Antibody (OTI13H11) - Azide and BSA Free [NBP2-72919] - Analysis of extracts (10ug) from 6 different cell lines by using NDUFB9 monoclonal antibody.

Flow Cytometry: NDUFB9 Antibody (OTI13H11) - Azide and BSA Free [NBP2-72919] - Analysis of Hela cells, using NDUFB9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI13H11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NDUFB9 Antibody (OTI13H11) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 3-179 of human NDUFB9(NP_0049906) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDUFB9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:1000
• Theoretical MW: 21.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for NDUFB9 Antibody (OTI13H11) - Azide and BSA Free

• B22
• CI-B22
• complex I B22 subunit
• Complex I-B22
• FLJ22885
• LYR motif-containing protein 3
• LYRM3DKFZp566O173
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
• NADH-ubiquinone oxidoreductase B22 subunit
• UQOR22NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22kD, B22)

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFB9