NDUFB2 Antibody (S03-7G4)

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Western Blot: NDUFB2 Antibody (S03-7G4) [NBP3-19709] - Western blot detection of NDUFB2 in mouse spleen, rat heart, mouse heart using NBP3-19709. Predicted band size: 12kDa. Observed band size: 12kDa.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
S03-7G4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

NDUFB2 Antibody (S03-7G4) Summary

Description
Novus Biologicals Rabbit NDUFB2 Antibody (S03-7G4) (NBP3-19709) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human NDUFB2 (Uniprot # O95178)
Source
HEK293
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NDUFB2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1/1000-1/2000
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for NDUFB2 Antibody (S03-7G4)

  • CI-AGGGmitochondrial
  • MGC70788
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2 (8kD, AGGG)
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa

Background

The protein encoded by the NDUFB2 gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NDUFB2